Determination of Glutathione and Related Aminothiols in Mouse Tissues by Gas Chromatography with Flame Photometric Detection | Zendy

Kiyomi Takagi | Zendy; Hiroyuki Kataoka | Zendy; Masami Makita | Zendy

Open Access

Determination of Glutathione and Related Aminothiols in Mouse Tissues by Gas Chromatography with Flame Photometric Detection

Author(s) -

Kiyomi Takagi,

Hiroyuki Kataoka,

Masami Makita

Publication year - 1996

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60.729

Subject(s) - chromatography , chemistry , gas chromatography

A selective and sensitive method for the determination of glutathione and such related aminothiols as Cys, cysteinylglycine and gamma-glutamylcysteine in mouse tissues by gas chromatography was developed. After fractionating a tissue sample into the free and protein-bound fractions, glutathione and aminothiols in each fraction were converted into their N,S-isopropoxycarbonyl methyl ester derivatives and then measured by gas chromatography with flame photometric detection, using a DB-1 capillary column. This method enabled the redox status of glutathione and related aminothiols in mouse tissues to be investigated.

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