Purification and Characterization of New Trehalose-producing Enzymes Isolated from the Hyperthermophilic Archae,Sulfolobus solfataricusKM1 | Zendy

Masaru Kato | Zendy; Yutaka Miura | Zendy; Masako Kettoku | Zendy; Kazutoshi Shindo | Zendy; Akihiro Iwamatsu | Zendy; Kazuo Kobayashi | Zendy

Open Access

Purification and Characterization of New Trehalose-producing Enzymes Isolated from the Hyperthermophilic Archae,Sulfolobus solfataricusKM1

Author(s) -

Masaru Kato,

Yutaka Miura,

Masako Kettoku,

Kazutoshi Shindo,

Akihiro Iwamatsu,

Kazuo Kobayashi

Publication year - 1996

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60.546

Subject(s) - sulfolobus solfataricus , sulfolobus , trehalose , enzyme , biochemistry , chemistry , archaea , gene

Amylolytic activity that converts soluble starch to alpha, alpha-trehalose (trehalose), was found in the cell homogenate of the hyperthermophilic acidophilic archae, Sulfolobus solfataricus KM1. DEAE chromatography of the homogenate as well as other new reliable assay methods showed two enzymes to be essential for this activity. These enzymes, a glycosyltransferase and an amylase, were purified to homogeneity and characterized. Their molecular masses were 76 kDa and 61 kDa and activities were maximal at 70-80 degrees C and 70-85 degrees C, respectively. High thermostability was noted for each. The reaction products by the two enzymes on maltooligosaccharides were identified by 1H- and 13C-NMR spectra and HPLC analysis. The cooperative mechanism of the two enzymes was used in a new enzymatic pathway for trehalose synthesis from starch.

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