Thymine Dimer-dependent Incision on Ultraviolet Light Damaged-DNA in Cell-free Extracts ofChlorella pyrenoidosa | Zendy

Chih-Wei Chang | Zendy; Jhon-Chun Ho | Zendy; Todd Hsu | Zendy

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Thymine Dimer-dependent Incision on Ultraviolet Light Damaged-DNA in Cell-free Extracts ofChlorella pyrenoidosa

Author(s) -

Chih-Wei Chang,

Jhon-Chun Ho,

Todd Hsu

Publication year - 1996

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60.490

Subject(s) - chlorella pyrenoidosa , pyrimidine dimer , thymine , dna , dimer , oligonucleotide , agarose gel electrophoresis , chemistry , biochemistry , gel electrophoresis , ultraviolet light , biology , microbiology and biotechnology , algae , chlorella , dna damage , photochemistry , botany , organic chemistry

Cell-free extracts of the unicellular algae Chlorella pyrenoidosa induce a UV-dependent incision of both phi x 174 RFI DNA and a 27-mer double-stranded oligonucleotide containing a single thymine dimer, as analyzed by agarose and denaturing polyacrylamide gel electrophoresis, respectively. The incision reaction occurred only on the thymine dimer-containing strand of the 27-mer, and a major incision product of 17-mer was observed. Addition of ATP or ATP-regenerating system to algal extracts increased the effect of DNA incision.

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