The Fate of Mn2+Ions InsideSaccharomyces cerevisiaeCells Seen by Electron Paramagnetic Resonance
Author(s) -
Ileana C. Fărcăşanu,
Nobuaki Ohta,
Tokichi Miyakawa
Publication year - 1996
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.60.468
Subject(s) - electron paramagnetic resonance , manganese , ion , saccharomyces cerevisiae , chemistry , resonance (particle physics) , spectral line , nuclear magnetic resonance , analytical chemistry (journal) , yeast , atomic physics , physics , biochemistry , chromatography , organic chemistry , astronomy
The fate of Mn2+ inside Saccharomyces cerevisiae cells was monitored during import and export processes, using information from electron paramagnetic resonance (EPR) spectroscopy analysis. EPR spectra showed that when entering the cell in high number, manganese ions immediately precipitated. If recorded under conditions that favored manganese efflux, EPR spectra indicated that only osmotically free ions were exported and that bound manganese had to turn into the soluble form before being able to leave the cell.
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