Cluster Analysis by Measurement of Peroxidase and Esterase fromCitrusFlavedo
Author(s) -
Xiao Hong Zheng,
M. Nishioka,
Akiko Kawamura,
Hiroyuki Ukeda,
Masayoshi Sawamura,
Hirozo Kusunose
Publication year - 1996
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.60.390
Subject(s) - point of delivery , cultivar , esterase , horticulture , peroxidase , botany , polyacrylamide gel electrophoresis , densitometer , biology , enzyme , biochemistry , physics , quantum mechanics
The zymograms of peroxidase (POD) and esterase (EST) from seventy-two kinds of Citrus fruits and the fruit of both Poncirus and Fortunella were obtained by polyacrylamide gel electrophoresis. It was found that there was little variance in the band pattern of POD and EST of the fruits harvested after September. There were at least three bands of POD and approximately ten of EST in each cultivar. The density of each band for EST was measured with a densitometer. There was a characteristic band for POD identifying pummelos. The band patterns of EST were also characteristic among each species or cultivar. Two kinds of ootachibana (Citrus otachibana Hort. ex Tanaka) were shown to be different cultivars from each other according to their EST zymograms and peel oil analysis. Cluster analysis based on EST was done and discussed together with POD.
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