N-Linked Sugar Chain of 55-kDa Royal Jelly Glycoprotein | Zendy

Yoshinobu Kimura | Zendy; Shin-ichiro Kajiyama | Zendy; Jun Kanaeda | Zendy; Tomomi Izukawa | Zendy; Masami Yonekura | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

N-Linked Sugar Chain of 55-kDa Royal Jelly Glycoprotein

Author(s) -

Yoshinobu Kimura,

Shin-ichiro Kajiyama,

Jun Kanaeda,

Tomomi Izukawa,

Masami Yonekura

Publication year - 1996

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60.2099

Subject(s) - sugar , mannose , glycoprotein , chemistry , biochemistry , acetylation , carbohydrate , stereochemistry , gene

An N-linked sugar chain from 55-kDa royal jelly glycoprotein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507-509 (1995)], has been identified. The sugar chains were released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential exo-mannosidase digestions, MALDI-TOF MS, and 500 MHz 1H-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Man alpha 1-->2Man alpha 1-->6(Man alpha 1--> 2Man alpha 1-->3)Man alpha 1-->6(Man alpha 1-->2Man alpha 1-->2Man alpha 1-->3)Man beta 1-->4GlcNAc beta 1-->4GlcNAc, which is a non-processed high mannose type structure.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research