Gene Cloning and Expression of New Trehalose-producing Enzymes from the Hyperthermophilic ArchaeumSulfolobus solfataricusKM1 | Zendy

Kazuo Kobayashi | Zendy; Masaru Kato | Zendy; Yutaka Miura | Zendy; Masako Kettoku | Zendy; Toshihiro Komeda | Zendy; Akihiro Iwamatsu | Zendy

Open Access

Gene Cloning and Expression of New Trehalose-producing Enzymes from the Hyperthermophilic ArchaeumSulfolobus solfataricusKM1

Author(s) -

Kazuo Kobayashi,

Masaru Kato,

Yutaka Miura,

Masako Kettoku,

Toshihiro Komeda,

Akihiro Iwamatsu

Publication year - 1996

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60.1882

Subject(s) - sulfolobus solfataricus , trehalose , biochemistry , sulfolobus , glycosyltransferase , glycosyl , gene , nucleic acid sequence , enzyme , biology , amino acid , hyperthermophile , archaea

The genes encoding for trehalose-producing enzymes, a glycosyl-trehalose-producing enzyme (glycosyltransferase) and a gylcosyl-trehalose-hydrolyzing enzyme (alpha-amylase), from Sulfolobus solfataricus KM1 were cloned and expressed in E. coli. The nucleotide sequence of the glycosyltransferase gene and the alpha-amylase gene indicated proteins with lengths of 728 and 558 amino acids and molecular masses of 86-kDa and 65-kDa, respectively. Regions highly conserved in the alpha-amylase family exist in the amino acid sequences of these enzymes.

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