Sequence-specific Binding Sites in the Taka-amylase A G2 Promoter for the CreA Repressor Mediating Carbon Catabolite Repression | Zendy

Masashi Kato | Zendy; Kotaro Sekine | Zendy; Norihiro Tsukagoshi | Zendy

Open Access

Sequence-specific Binding Sites in the Taka-amylase A G2 Promoter for the CreA Repressor Mediating Carbon Catabolite Repression

Author(s) -

Masashi Kato,

Kotaro Sekine,

Norihiro Tsukagoshi

Publication year - 1996

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60.1776

Subject(s) - catabolite repression , repressor , aspergillus oryzae , psychological repression , binding site , aspergillus nidulans , biology , gene , microbiology and biotechnology , promoter , maltose binding protein , escherichia coli , transcription (linguistics) , biochemistry , fusion protein , transcription factor , gene expression , enzyme , mutant , recombinant dna , linguistics , philosophy

The N-terminal part of the CreA protein encompassing two zinc fingers was expressed in Escherichia coli as a fusion protein with the maltose binding protein (MalE) of E. coli. Our results show that CreA binds to the promoter of the Taa-G2 gene encoding Taka-amylase A of Aspergillus oryzae. DNase I footprinting experiments showed that CreA bound to three sites with high affinity and to one site with low affinity within the first 401-bp region upstream of the transcription initiation site. All of the sites contained sequences related to the CreA consensus binding site (5'-SYGGRG-3'), and are suggested to participate in repression of the Taa-G2 gene in response to glucose.

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