Gene Analysis of Trehalose-producing Enzymes from Hyperthermophilic Archaea inSulfolobales
Author(s) -
Kazuo Kobayashi,
Masaru Kato,
Yutaka Miura,
Masako Kettoku,
Toshihiro Komeda,
Akihiro Iwamatsu
Publication year - 1996
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.60.1720
Subject(s) - trehalose , archaea , gene , sulfolobus acidocaldarius , enzyme , biology , sulfolobus solfataricus , biochemistry , genetics
The genes encoding new trehalose-producing enzymes from S. acidocaldarius ATCC33909 were cloned to analyze the distribution of these genes in Sulfolobales. Comparison of the amino acid sequences with S. solfataricus KM1 showed approximately 50% similarity. Southern analysis suggest that homologues of the trehalose-producing enzyme genes exist widely in sulfolobales and strains in Sulfolobales were classified into three kinds of genotypes.
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