Molecular Cloning of a Genomic DNA for Enolase fromAspergillus oryzae | Zendy

Masayuki Machida | Zendy; Trini Violeta Jime’nez Gonzalez | Zendy; Lim Kong Boon | Zendy; Katsuya Gomi | Zendy; Yoshifumi Jigami | Zendy

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Molecular Cloning of a Genomic DNA for Enolase fromAspergillus oryzae

Author(s) -

Masayuki Machida,

Trini Violeta Jime’nez Gonzalez,

Lim Kong Boon,

Katsuya Gomi,

Yoshifumi Jigami

Publication year - 1996

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60.161

Subject(s) - aspergillus oryzae , genomic dna , gene , bglii , homology (biology) , biology , genetics , intron , enolase , microbiology and biotechnology , genome , biochemistry , enzyme , immunohistochemistry , bamhi , immunology

We have isolated an enolase gene (enoA) from Aspergillus oryzae by heterologous hybridization using the corresponding Saccharomyces cerevisiae ENO2 gene as a probe. A 2.9-kb BglII-fragment contained the entire structural gene enoA including 5'- and 3'- flanking regions. The homology between A. oryzae enoA and S. cerevisiae ENO2 genes is 66.9% when introns are removed. Genomic Southern analysis indicated that there is only one enolase gene in A. oryzae.

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