Production of Fructosyl Lysine Oxidase fromFusarium oxysporumS-1F4 on Autoclave-browned Medium | Zendy

Yasuyoshi Sakai | Zendy; Nobuyuki Yoshida | Zendy; Yoshiki Tani | Zendy; Nobuo Kato | Zendy

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Production of Fructosyl Lysine Oxidase fromFusarium oxysporumS-1F4 on Autoclave-browned Medium

Author(s) -

Yasuyoshi Sakai,

Nobuyuki Yoshida,

Yoshiki Tani,

Nobuo Kato

Publication year - 1996

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60.150

Subject(s) - lysine , amadori rearrangement , browning , cycloheximide , chemistry , enzyme , glycation , biochemistry , autoclave , fusarium oxysporum , food science , protein biosynthesis , biology , botany , amino acid , organic chemistry , receptor

In Fusarium oxysporum S-1F4, fructosyl lysine oxidase (FLOD) was induced with N epsilon-fructosyl N alpha-Z-lysine (epsilon-FL), which is a model compound of a glycated protein, and the induction was inhibited by the addition of cycloheximide in the growing cells. FLOD formation was greatly enhanced in an autoclave-browned medium containing glucose and L-lysine. Some Amadori compounds formed from glucose and L-lysine during autoclaving were assumed to induce the enzyme. After optimization of the culture conditions, FLOD produced in the browned medium was comparable to that in the medium with epsilon-FL.

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