Open AccessPrimary Structure of an Allergenic Peptide Occurring in the Chymotryptic Hydrolysate of Gluten
Author(s) -
Michiko Watanabe,
Soichi Tanabe,
Takashi Suzuki,
Zenrō Ikezawa,
Soichi Arai
Publication year - 1995
Publication title -
bioscience, biotechnology, and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.59.1596
Subject(s) - hydrolysate , peptide , glutenin , chemistry , gluten , size exclusion chromatography , protein primary structure , biochemistry , peptide sequence , wheat gluten , amino acid , hydrolysis , enzyme , protein subunit , gene
An allergenic peptide was isolated from the chymotryptic hydrolysate of gluten by gel filtration and HPLC. The primary structure of the peptide was determined as Ser-Gln-Gln-Gln-Gln- Pro-Pro-Phe-Ser-Gln-Gln-Gln-Pro-Pro-Phe-Ser-Gln-Gln-Gln-Gln-Pro-Pro-Phe- Ser- Gln-Gln-Gln-Gln-Pro-Pro-Phe-Ser-Gln-Gln-Gln-Pro-Pro-Phe. The amino acid sequence similarity shows that the peptide originated from a low-molecular-weight glutenin chain.