Open AccessEfficient Expression of a Transfected Foreign Gene by Cos1 Cells in Serum-free Medium
Author(s) -
Hideya Ohashi,
Tomoko Sudo
Publication year - 1994
Publication title -
bioscience, biotechnology, and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.58.758
Subject(s) - transfection , bovine serum albumin , fetal bovine serum , gene expression , chemistry , microbiology and biotechnology , gene , biology , cell , biochemistry
In this report, we have found that fatty acid-free bovine serum albumin stimulated the expression of a transfected foreign gene in Cos1 cells in serum-free medium and that its activity was as same as that of fetal calf serum. This will simplify the purification of the gene product from the culture medium.