Open AccessAn Extracellular Quinoprotein Oxidase That Catalyzes Conversion of Enacyloxin IVa to Enacyloxin IIa
Author(s) -
Ryo Oyama,
Toshihiko Watanabe,
Hiroko Hanzawa,
Taketo Sano,
Takeyoshi Sugiyama,
Kazuo Izaki
Publication year - 1994
Publication title -
bioscience, biotechnology, and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.58.1914
Subject(s) - extracellular , chemistry , biochemistry
A new extracellular quinoprotein oxidase named enacyloxin oxidase (ENX oxidase), which is involved in biosynthesis of ENX IIa, a congener of ENX, was found in the culture supernatant of Frateuria sp. W-315 and purified as a homogeneous protein on SDS-PAGE. ENX oxidase was shown to have a molecular mass of 73 kDa by SDS-PAGE and 79 kDa by gel filtration. The enzyme was inhibited by various carbonyl reagents and the activity was stimulated by addition of PQQ. This is the first report on a quinoprotein oxidase that is secreted into the culture medium in the logarithmic growth phase, and acts for biosynthesis of the antibiotic.