Open AccessMetabolic Engineering for Production ofβ-Carotene and Lycopene inSaccharomyces cerevisiae
Author(s) -
Shigeyuki Yamano,
Takeaki Ishii,
Masaya Nakagawa,
Hiroshi Ikenaga,
Norihiko Misawa
Publication year - 1994
Publication title -
bioscience, biotechnology, and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.58.1112
Subject(s) - lycopene , saccharomyces cerevisiae , carotenoid , metabolic engineering , yeast , ergosterol , biosynthesis , carotene , biochemistry , chemistry , gene , biology , food science
We have engineered a conventional yeast, Saccharomyces cerevisiae, to confer a novel biosynthetic pathway for the production of beta-carotene and lycopene by introducing the bacterial carotenoid biosynthesis genes, which are individually surrounded by the promoters and terminators derived from S. cerevisiae. beta-Carotene and lycopene accumulated in the cells of this yeast, which was considered to be a result of the carbon flow for the ergosterol biosynthetic pathway being partially directed to the pathway for the carotenoid production.