Expression of an 87-kD-β-1,3-Glucanase ofBacillus circulansIAM1165 inSaccharomyces cerevisiaeby Low-temperature Incubation | Zendy

Harushi Nakajima | Zendy; Kasumi Noguchi | Zendy; Mami Yamamoto | Zendy; Rikizo Aono | Zendy; Koki Horikoshi | Zendy

Open Access

Expression of an 87-kD-β-1,3-Glucanase ofBacillus circulansIAM1165 inSaccharomyces cerevisiaeby Low-temperature Incubation

Author(s) -

Harushi Nakajima,

Kasumi Noguchi,

Mami Yamamoto,

Rikizo Aono,

Koki Horikoshi

Publication year - 1993

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.57.2039

Subject(s) - bacillus circulans , library science , biology , computer science , genetics , bacteria

A DNA segment encoding a signal peptide from yeast invertase was fused in frame to bglH gene encoding 87-kD-beta-1,3-glucanase from Bacillus circulans IAM1165 and was expressed in the yeast Saccharomyces cerevisiae under the control of the GAL1 gene promoter. Yeast cells containing this fused gene produced active beta-1,3-glucanase in the medium after a long period of incubation at low temperature. The enzyme produced by yeast was heterogeneous in size, and larger than the enzyme produced by Escherichia coli.

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