Open AccessCloning and Nucleotide Sequences of Two Genes Involved in the 4″-O-Acylation of Macrolide Antibiotics fromStreptomyces thermotolerans
Author(s) -
Akira Arisawa,
Naoto Kawamura,
Hiroshi Tsunekawa,
Koji Okamura,
Hiroshi Tone,
Rokuro Okamoto
Publication year - 1993
Publication title -
bioscience, biotechnology, and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.57.2020
Subject(s) - corporation , acylation , library science , political science , computer science , chemistry , law , biochemistry , catalysis
A DNA fragment responsible for the 4''-O-acylation of macrolide antibiotics was cloned from a mutant strain of the carbomycin producer Streptomyces thermotolerans. The gene encoding the macrolide 4''-O-acyltransferase was within a 2.7-kb region of the cloned fragment (15-kb). Streptomyces lividans carrying the region converted exogenously added tylosin to 4''-O-acyltylosins. Nucleotide sequencing of the region showed two open reading frames (ORFs). Expression assay using deleted plasmids showed that both ORFs were essential for optimal expression of the acyltransferase activity. One of them (acyB1) was identical with carE reported previously as a gene encoding 4''-mycarosyl isovaleryl-CoA transferase. The other (acyB2) was assumed to encode a novel regulatory protein that could active acyB1 expression. acyB1 and acyB2 were highly conserved among streptomycetes with macrolide 4''-O-acyl transferase activity.