Physical Mapping of Multiple Homologous Genes in theBacillus subtilis168 Chromosome: Identification of Ten Ribosomal RNA Operon Loci | Zendy

Mitsuhiro Itaya | Zendy

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Physical Mapping of Multiple Homologous Genes in theBacillus subtilis168 Chromosome: Identification of Ten Ribosomal RNA Operon Loci

Author(s) -

Mitsuhiro Itaya

Publication year - 1993

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.57.1611

Subject(s) - operon , genetics , bacillus subtilis , biology , ribosomal rna , homologous chromosome , chromosome , gene , restriction map , microbiology and biotechnology , escherichia coli , plasmid , bacteria

Ten ribosomal RNA operons (rrn) of the Bacillus subtilis 168 strain were precisely located in the complete physical map, i.e., the NotI and SfiI restriction map [M. Itaya and Tanaka, J. Mol. Biol., 220, 631-648 (1991)]. Mapping was done by identification of the NotI and the SfiI fragments to which the rrnO operon hybridized. This protocol proved that homologous multiple genes dispersed on the chromosome can be physically mapped by using a single membrane filter. A previously unidentified deletion formation was shown to be that caused by homologous recombination between rrnJ-W.

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