A Novel and Efficient Method for Enzymatic Synthesis of High Purity Maltose Using Moranoline (1-Deoxynojirimycin) | Zendy

Shigeaki Maruo | Zendy; Hiroshi Yamashita | Zendy; Katsunori Miyazaki | Zendy; Hirofumi Yamamoto | Zendy; Yohsuke Kyotani | Zendy; H. Ogawa | Zendy; Masahiko Kojima | Zendy; Yohji Ezure | Zendy

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A Novel and Efficient Method for Enzymatic Synthesis of High Purity Maltose Using Moranoline (1-Deoxynojirimycin)

Author(s) -

Shigeaki Maruo,

Hiroshi Yamashita,

Katsunori Miyazaki,

Hirofumi Yamamoto,

Yohsuke Kyotani,

H. Ogawa,

Masahiko Kojima,

Yohji Ezure

Publication year - 1992

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.56.1406

Subject(s) - chemistry , library science , engineering , computer science

A transglycosylation reaction with moranoline (1-deoxynojirimycin) was done with soluble starch as the glucosyl donor and Bacillus macerans amylase as a cyclodextrin glycosyltransferase [EC 2.4.1.19]. The resultant transglycosylation products with moranoline, obtained by treating the reaction mixture with a strong cation exchange resin, were hydrolyzed by beta-amylase [EC 3.2.1.2] from sweet potatoes. The hydrolysate was treated with a strong cation exchange resin, and high purity maltose was obtained.

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