Open AccessPurification and Some Properties of Acidocin 8912, a Novel Bacteriocin Produced byLactobacillus acidophilusTK8912
Author(s) -
Takatsugu Tahara,
Kazuo Kanatani,
Kazushi Yoshida,
Hirosumi Miura,
Masaru Sakamoto,
Masao Oshimura
Publication year - 1992
Publication title -
bioscience, biotechnology, and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.56.1212
Subject(s) - brewing , lactobacillus acidophilus , library science , chemistry , biology , food science , computer science , bacteria , probiotic , fermentation , genetics
Acidocin 8912, a bacteriocin produced by Lactobacillus acidophilus TK8912, was purified by ammonium sulfate fractionation and successive chromatographies on CM-cellulose, Sephadex G-50, Sephadex G-25, and reversed-phase HPLC on Aquapore RP-300. The purified acidocin 8912 migrated as a single band on SDS-PAGE. The molecular weight was estimated to be 5200 by SDS-PAGE, and 5400 by HPLC gel filtration on TSKgel G3000PWXL. Both the amino acid composition and the N-terminal amino acid sequence analysis indicated that acidocin 8912 was a peptide composed of presumably 50 amino acids containing a Lys residue at the N-terminus. The purified acidocin 8912 showed a bactericidal effect on sensitive cells but not a bacteriolytic effect.