Biosynthesis and Hydrolysis of Poly(γ-glutamic acid) fromBacillus subtilisIF03335 | Zendy

Atsuo Goto | Zendy; Masao Kunioka | Zendy

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Biosynthesis and Hydrolysis of Poly(γ-glutamic acid) fromBacillus subtilisIF03335

Author(s) -

Atsuo Goto,

Masao Kunioka

Publication year - 1992

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.56.1031

Subject(s) - bacillus subtilis , glutamic acid , hydrolysis , citric acid , chemistry , ammonium sulfate , acid hydrolysis , ammonium , aqueous solution , biochemistry , biosynthesis , organic chemistry , amino acid , nuclear chemistry , bacteria , enzyme , biology , genetics

Poly(γ-glutamic acid) (PGA) production in Bacillus subtilis IF03335 was studied. When citric acid as a carbon source was added to a glutamic acid medium containing L-glutamic acid and ammonium sulfate, a large amount of pure PGA was produced. On the other hand, when glucose was added to the glutamic acid medium, a by-product was produced, which seemed to be a polysaccharide. Moreover, the mode of hydrolysis was investigated with PGA in aqueous solutions at 80, 100, and 120°C by monitoring the time-dependent changes in the molecular weights. Hydrolytic degradation of PGA was found to proceed through a random chain scission.

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