A Mucus Adhesion Promoting Protein, MapA, Mediates the Adhesion ofLactobacillus reuterito Caco-2 Human Intestinal Epithelial Cells | Zendy

Yukihiro MIYOSHI | Zendy; Sanae Okada | Zendy; Tai Uchimura | Zendy; Eiichi Satoh | Zendy

Open Access

A Mucus Adhesion Promoting Protein, MapA, Mediates the Adhesion ofLactobacillus reuterito Caco-2 Human Intestinal Epithelial Cells

Author(s) -

Yukihiro MIYOSHI,

Sanae Okada,

Tai Uchimura,

Eiichi Satoh

Publication year - 2006

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.50688

Subject(s) - lactobacillus reuteri , adhesion , caco 2 , mucus , microbiology and biotechnology , receptor , bacteria , gastrointestinal tract , biology , chemistry , in vitro , lactobacillus , biochemistry , genetics , ecology , organic chemistry

Lactobacillus reuteri is one of the dominant lactobacilli found in the gastrointestinal tract of various animals. A surface protein of L. reuteri 104R, mucus adhesion promoting protein (MapA), is considered to be an adhesion factor of this strain. We investigated the relation between MapA and adhesion of L. reuteri to human intestinal (Caco-2) cells. Quantitative analysis of the adhesion of L. reuteri strains to Caco-2 cells showed that various L. reuteri strains bind not only to mucus but also to intestinal epithelial cells. In addition, purified MapA bound to Caco-2 cells, and this binding inhibited the adhesion of L. reuteri in a concentration-dependent manner. Based on these observations, the adhesion of L. reuteri appears due to the binding of MapA to receptor-like molecules on Caco-2 cells. Further, far-western analysis indicated the existence of multiple receptor-like molecules in Caco-2 cells.

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