Chicken Ovalbumin Promoter Is Demethylated upon Expression in the Regions Specifically Involved in Estrogen-Responsiveness | Zendy

Mahboob Morshed | Zendy; Shusuke Sano | Zendy; Daisuke Nishimiya | Zendy; Munetoshi Ando | Zendy; Kenichi Nishijima | Zendy; Shinji Iijima | Zendy

Open Access

Chicken Ovalbumin Promoter Is Demethylated upon Expression in the Regions Specifically Involved in Estrogen-Responsiveness

Author(s) -

Mahboob Morshed,

Shusuke Sano,

Daisuke Nishimiya,

Munetoshi Ando,

Kenichi Nishijima,

Shinji Iijima

Publication year - 2006

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.50682

Subject(s) - ovalbumin , microbiology and biotechnology , dna methylation , methylated dna immunoprecipitation , chromatin immunoprecipitation , biology , histone , chromatin , oviduct , methylation , dna , cpg site , hypersensitive site , histone h3 , epigenetics , chemistry , promoter , gene , gene expression , biochemistry , genetics , antigen , endocrinology

Here we report the methylation status of the chicken ovalbumin promoter. Genomic DNA of oviduct from immature chickens and laying hens was analyzed through bisulfite genomic sequencing. In the ovalbumin control locus up to the 6 kb upstream region, CpG sites were methylated in immature chickens, except for several sites, and almost all CpGs residing in DNase I hypersensitive sites I, II, and III, but not IV, were selectively unmethylated in ovalbumin expressing chickens. Chromatin immunoprecipitation assays showed that the ovalbumin control region was associated with acetylated histone H3 but not with dimethylated histone H3 at Lys 27. These results demonstrate that DNA demethylation was restricted to short DNA regions of DNase I hypersensitive sites, especially to those which participated in estrogen-responsiveness, even when cells expressed extremely high levels of ovalbumin and these sites were associated with acetylated histones.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research