The White-Rot FungusPleurotus ostreatusTransformant Overproduced Intracellular cAMP and Laccase | Zendy

Yuki Yao | Zendy; Takaiku Sakamoto | Zendy; Yoichi Honda | Zendy; Yasuyuki Kagotani | Zendy; Kousuke Izumitsu | Zendy; Kazumi Suzuki | Zendy; Toshikazu Irie | Zendy

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The White-Rot FungusPleurotus ostreatusTransformant Overproduced Intracellular cAMP and Laccase

Author(s) -

Yuki Yao,

Takaiku Sakamoto,

Yoichi Honda,

Yasuyuki Kagotani,

Kousuke Izumitsu,

Kazumi Suzuki,

Toshikazu Irie

Publication year - 2013

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.130470

Subject(s) - pleurotus ostreatus , laccase , intracellular , transformation (genetics) , ferulic acid , biology , heterotrimeric g protein , biochemistry , microbiology and biotechnology , mycelium , fungus , chemistry , enzyme , gene , botany , g protein , receptor , mushroom

Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO4 and ferulic acid.

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