Bioconversion of Phosphatidylserine by Phospholipase D fromStreptomyces racemochromogenesin a Microaqueous Water-Immiscible Organic Solvent | Zendy

Sheng Chen | Zendy; Lin Xu | Zendy; Yan Li | Zendy; Ning Hao | Zendy; Ming Yan | Zendy

Open Access

Bioconversion of Phosphatidylserine by Phospholipase D fromStreptomyces racemochromogenesin a Microaqueous Water-Immiscible Organic Solvent

Author(s) -

Sheng Chen,

Lin Xu,

Yan Li,

Ning Hao,

Ming Yan

Publication year - 2013

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.130388

Subject(s) - bioconversion , hydrolysis , chemistry , solvent , phosphatidylcholine , organic solvent , phospholipase d , phosphatidylserine , organic chemistry , chromatography , medicinal chemistry , nuclear chemistry , enzyme , biochemistry , phospholipid , chemical engineering , fermentation , membrane , engineering

Phospholipase D (PLD)-mediated transphosphatidylation of phosphatidylcholine (PC) in a biphasic system was limited by the hydrolysis reaction. A biphasic system can produce a large amount of water. To solve this problem, a microaqueous water-immiscible organic solvent was used for the first time in the bioconversion of phosphatidylserine (PS). The transphosphatidylation among 40 µmol PC, 800 µmol L-serine, and 0.17 U/mL PLD in 2.133 mL of butyl acetate with 6.25% water (V/V) was conducted at a trans-phosphatidylation rate of 88% (mol/mol), and no hydrolytic reaction was observed. Compared to commonly used biphasic systems, this system shows a similar transphosphatidylation rate, whereas the undesirable hydrolysis of phospholipids was completely suppressed.

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