Functional Expression ofSchizosaccharomyces pombeVba2p in the Vacuolar Membrane ofSaccharomyces cerevisiae | Zendy

Pongsanat Pongcharoen | Zendy; Miyuki KawanoKawada | Zendy; Tomoko Iwaki | Zendy; Naoko Sugimoto | Zendy; Takayuki Sekito | Zendy; Koichi Akiyama | Zendy; Kaoru Takegawa | Zendy; Yoshimi Kakinuma | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Functional Expression ofSchizosaccharomyces pombeVba2p in the Vacuolar Membrane ofSaccharomyces cerevisiae

Author(s) -

Pongsanat Pongcharoen,

Miyuki KawanoKawada,

Tomoko Iwaki,

Naoko Sugimoto,

Takayuki Sekito,

Koichi Akiyama,

Kaoru Takegawa,

Yoshimi Kakinuma

Publication year - 2013

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.130387

Subject(s) - schizosaccharomyces pombe , saccharomyces cerevisiae , lysine , vacuole , yeast , quinidine , biochemistry , membrane , vesicle , schizosaccharomyces , chemistry , microbiology and biotechnology , amino acid , biology , cytoplasm , pharmacology

A vacuolar membrane protein, Vba2p of Schizosaccharomyces pombe, is involved in basic amino acid uptake by intact cells. Here we found evidence that Vba2p mediated ATP-dependent lysine uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae. Vba2p was also responsible for quinidine sensitivity, and the addition of lysine improved cell growth on quinidine-containing media. These findings should be useful for further characterization of Vba2p.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research