The UDP-N-acetylglucosamine:Dolichol Phosphate-N-acetylglucosamine-phosphotransferase Gene as a New Selection Marker for Potato Transformation | Zendy

HyunSoon Kim | Zendy; Mi-Sun Kim | Zendy; Kyoung-A HWANG | Zendy; Se-Won PARK | Zendy; Jae-Heung Jeon | Zendy

Open Access

The UDP-N-acetylglucosamine:Dolichol Phosphate-N-acetylglucosamine-phosphotransferase Gene as a New Selection Marker for Potato Transformation

Author(s) -

HyunSoon Kim,

Mi-Sun Kim,

Kyoung-A HWANG,

Se-Won PARK,

Jae-Heung Jeon

Publication year - 2013

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.130146

Subject(s) - tunicamycin , transformation (genetics) , dolichol , phosphotransferase , biology , n acetylglucosamine , genetically modified crops , gene , biochemistry , transgene , microbiology and biotechnology , biosynthesis , enzyme , unfolded protein response

Here we describe the generation of potato plants that constitutively overexpressed, UDP-N-acetylglucosamine:dolichol phosphate-N-acetylglucosamine-phosphotransferase (GPT). Such transgenic plants can be formed in a medium with tunicamycin at 9.8 ± 0.28% efficiency, similar to the 9.4 ± 1.10 for the bialaphos resistance gene (Bar) gene. This study indicated that GPT transformation was very stable with high reproducibility, and that growth and tuber production in the GPT-transformed plants were stronger than in the wild-type plants.

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