Glycosaminoglycan and Collagen Facilitate the Degradation of Pigment Epithelium-Derived Factor by Chymotrypsin | Zendy

Akio Saito | Zendy

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Glycosaminoglycan and Collagen Facilitate the Degradation of Pigment Epithelium-Derived Factor by Chymotrypsin

Author(s) -

Akio Saito

Publication year - 2013

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.130069

Subject(s) - dermatan sulfate , chemistry , plasmin , keratan sulfate , biochemistry , pedf , glycosaminoglycan , heparin , kallikrein , chymotrypsin , chondroitin sulfate , cathepsin g , heparan sulfate , thrombin , proteases , heparin cofactor ii , trypsin , enzyme , biology , platelet , immunology , retinal , antithrombin

Pigment epithelium-derived factor (PEDF) is a member of the serine protease inhibitor family. It is present in a variety of tissues and organs, including plasma. Here, PEDF was purified from human plasma by use of a dermatan sulfate affinity column, and then hydroxyapatite, gel filtration and ion exchange columns. It did not form a complex with various proteases, including chymotrypsin, elastase, kallikrein, thrombin, plasmin, cathepsins G, activated protein C, and urokinase, but collagen type I facilitated the degradation of PEDF by chymotrypsin more than 10-fold. Dermatan sulfate, heparan sulfate, and heparin showed similar effects, but other glycosaminoglycans, such as hyaluronic acid, chondroitin sulfate A, C, D, E, and keratan sulfate, had no effect on PEDF degradation.

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