Aqueous Extracts ofRhizopus oryzaeInduced Nitric Oxide Production in Rat Hepatocyte Cell Line RLN-10 | Zendy

Takehito Suzuki | Zendy; Mayuko UCHIDA | Zendy; Yuji Takeda | Zendy; C. Mori | Zendy; Atsushi Onuki | Zendy; Yoko Miyazaki | Zendy; Ken Onda | Zendy; Setsuo Ushikoshi | Zendy; Kotaro SHITORI | Zendy; Kazuaki Tanaka | Zendy; Hidetoshi Morita | Zendy; T. Takizawa | Zendy

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Aqueous Extracts ofRhizopus oryzaeInduced Nitric Oxide Production in Rat Hepatocyte Cell Line RLN-10

Author(s) -

Takehito Suzuki,

Mayuko UCHIDA,

Yuji Takeda,

C. Mori,

Atsushi Onuki,

Yoko Miyazaki,

Ken Onda,

Setsuo Ushikoshi,

Kotaro SHITORI,

Kazuaki Tanaka,

Hidetoshi Morita,

T. Takizawa

Publication year - 2013

Publication title -

bioscience biotechnology and biochemistry

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.120951

Subject(s) - nitric oxide , hepatocyte , microbiology and biotechnology , activator (genetics) , rhizopus oryzae , chemistry , nitric oxide synthase , nitrite , cell culture , superoxide , biochemistry , biology , in vitro , enzyme , gene , nitrate , genetics , organic chemistry , fermentation

Aqueous extracts of Rhizopus oryzae (Aq-ROU) have a broad range of physiological activity. Here we identified a new physiological effect of Aq-ROU in rat hepatocyte cell line RLN-10. Aq-ROU induced the accumulation of nitrite, a stable metabolite nitric oxide (NO), in cell culture medium and induced potent diaminofluorescein-FM diacetate staining in the cells. Real-time reverse transcriptase (RT)-PCR analysis showed marked inducible NO synthase gene expression. Additionally, markedly enhanced expression of p22(phox) and temporally increased expression of NADPH oxidase1 indicated that superoxide was produced. Nuclear translocation of nuclear factor-kappa (NF-κ) B p65 increased remarkably following Aq-ROU and following lipopolysaccharide treatment, a potent activator of NF-κB. Ammonium pyrrolidine-1-carbodithioate, an inhibitor of NF-κB, inhibited NO production following Aq-ROU treatment. Our data indicate that Aq-ROU induces NO production and potentially the production of superoxide, which may contribute to the broad range of physiological effects observed for Aq-ROU ingested by animals.

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