Measurement of Barley β-Glucan Concentration in the Plasma by Sandwich ELISA Using Rat Dectin-1 | Zendy

Shingo Hino | Zendy; Hikaru Sato | Zendy; Tsukasa Matsuda | Zendy; Tatsuya Morita | Zendy

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Measurement of Barley β-Glucan Concentration in the Plasma by Sandwich ELISA Using Rat Dectin-1

Author(s) -

Shingo Hino,

Hikaru Sato,

Tsukasa Matsuda,

Tatsuya Morita

Publication year - 2013

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.120788

Subject(s) - recombinant dna , glucan , immune system , body weight , chemistry , pharmacology , chromatography , immunology , medicine , biochemistry , gene

We used recombinant rat dectin-1 proteins to newly establish sandwich ELISA for determining barley β-glucan (BβG). The ELISA method had a working range of 15-4,000 µg/L. Plasma BβG was detectable up to 24 h after an intravenous administration of BβG (1 mg/kg of body weight) to rats. This method may be an effective tool for investigating the immune modulatory effects of BβG.

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