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RT-PCR- and MALDI-TOF Mass Spectrometry-Based Identification and Discrimination of Isoforms Homologous to Pufferfish Saxitoxin- and Tetrodotoxin-Binding Protein in the Plasma of Non-Toxic Cultured Pufferfish (Takifugu rubripes)
Author(s) -
Ryohei Tatsuno,
Kenichi Yamaguchi,
Tomohiro Takatani,
Osamu Arakawa
Publication year - 2013
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.120701
Subject(s) - takifugu rubripes , saxitoxin , tetrodotoxin , gene isoform , mass spectrometry , homologous chromosome , chemistry , biology , microbiology and biotechnology , toxin , biochemistry , fishery , fish <actinopterygii> , chromatography , gene , endocrinology
Four genes of Takifugu rubripes, tentatively designated Tr1-Tr4, encoding homologs of pufferfish saxitoxin- and tetrodotoxin-binding protein, were identified by BLAST search and 3'-RACE. RT-PCR and MALDI-TOF mass spectrometry allowed the identification and discrimination of Tr isoforms from the non-toxically cultured specimens. The expression of Tr1 and Tr3 mRNAs exclusively in the liver and the presence of their products as 120-kDa plasma proteins were confirmed.

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