Enzymatic Assay forD-Aspartic Acid UsingD-Aspartate Oxidase and Oxaloacetate Decarboxylase | Zendy

Shiro Kato | Zendy; Tomoyuki IKUTA | Zendy; Hisashi Hemmi | Zendy; Shoji Takahashi | Zendy; Yoshio Kera | Zendy; Tohru Yoshimura | Zendy

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Enzymatic Assay forD-Aspartic Acid UsingD-Aspartate Oxidase and Oxaloacetate Decarboxylase

Author(s) -

Shiro Kato,

Tomoyuki IKUTA,

Hisashi Hemmi,

Shoji Takahashi,

Yoshio Kera,

Tohru Yoshimura

Publication year - 2012

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.120477

Subject(s) - citrate synthase , biochemistry , chemistry , enzyme , lactic acid , d amino acid oxidase , aspartic acid , oxidase test , chromatography , bacteria , amino acid , biology , genetics

An enzymatic assay system for D-Asp was established using D-aspartate oxidase and oxaloacetate decarboxylase. In this system, D-Asp is converted to pyruvate, which is determined fluorometrically with 1,2-diamino-4,5-methylenedioxybenzene. This method makes possible D-Asp measurement at the micromolar level. The D-Asp contents of an edible brown alga, Hijika fusiforme, a lactic acid bacteria beverage, and pig testis were determined by the method.

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