Improvement of Gene Targeting inAspergillus nidulanswith Excess Non-Homologous Fragments | Zendy

Kazuaki MATSUHIRA | Zendy; Yasuo Itoh | Zendy

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Improvement of Gene Targeting inAspergillus nidulanswith Excess Non-Homologous Fragments

Author(s) -

Kazuaki MATSUHIRA,

Yasuo Itoh

Publication year - 2012

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.120456

Subject(s) - homologous chromosome , homologous recombination , gene , transformation (genetics) , aspergillus nidulans , biology , genetics , gene targeting , chromosome , microbiology and biotechnology , computational biology , mutant

Efficiency of gene targeting was increased more than 10-fold when an excess molar ratio of non-homologous fragments was added in the transformation of Aspergillus nidulans. In the targeted transformants, integration of such fragments into the host chromosome was a rare event. Hence, our approach proved practical in terms of producing successful targeting events without disturbing the host chromosomes.

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