Mevalonate-Dependent Enzymatic Synthesis of Amorphadiene Driven by an ATP-Regeneration System Using Polyphosphate Kinase | Zendy

Manami Shimane | Zendy; Yoshinori Sugai | Zendy; Ryosuke Kainuma | Zendy; Masahiro Natsume | Zendy; Hiroshi Kawaide | Zendy

Open Access

Mevalonate-Dependent Enzymatic Synthesis of Amorphadiene Driven by an ATP-Regeneration System Using Polyphosphate Kinase

Author(s) -

Manami Shimane,

Yoshinori Sugai,

Ryosuke Kainuma,

Masahiro Natsume,

Hiroshi Kawaide

Publication year - 2012

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.120177

Subject(s) - polyphosphate , biochemistry , enzyme , yield (engineering) , escherichia coli , chemistry , atp synthase , kinase , phosphate , gene , materials science , metallurgy

Polyphosphate kinase (PPK), which can regenerate ATP from ADP, was utilized in the mevalonate-dependent enzymatic synthesis of amorphadiene. The activity of PPK, cloned from Escherichia coli, was determined by (31)P-NMR. The yield from the PPK-catalyzed synthesis was 25%, 2.5 times higher than that without PPK. The (31)P-NMR analysis of the final reaction mixture indicated no accumulation of intermediates.

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