Labeling of Polyethylenimine with Fluorescent Dye to Image Nucleus, Nucleolus, and Chromosomes in Digitonin-Permeabilized HeLa Cells | Zendy

Masayuki Saito | Zendy; Hisato Saitoh | Zendy

Open Access

Labeling of Polyethylenimine with Fluorescent Dye to Image Nucleus, Nucleolus, and Chromosomes in Digitonin-Permeabilized HeLa Cells

Author(s) -

Masayuki Saito,

Hisato Saitoh

Publication year - 2012

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.120140

Subject(s) - polyethylenimine , nucleolus , hela , digitonin , fluorescence , microbiology and biotechnology , mitosis , biology , fluorescence microscope , chemistry , cell , biophysics , nucleus , biochemistry , transfection , gene , optics , physics , enzyme

The visualization of nuclear architecture, which changes dynamically depending on the physiological and the pathological situation, remains an important challenge. Here we report that exposure of fluoresceinisothiocyanate-labeled polyethylenimine (FITC-PEI) to digitonin-permeabilized cervical cancer HeLa cells enable rapid detection of the morphology of the nuclear rim, the nucleolus, and mitotic chromosomes. This simple detection strategy can aid in scientific investigation for both basic research and diagnostic purposes.

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