Molecular and Catalytic Properties of 2,4-Diacetylphloroglucinol Hydrolase (PhlG) from Pseudomonas sp. YGJ3 | Zendy

Hiroki SAITOU | Zendy; Manami Watanabe | Zendy; Kiyofumi MARUYAMA | Zendy

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Molecular and Catalytic Properties of 2,4-Diacetylphloroglucinol Hydrolase (PhlG) from <i>Pseudomonas</i> sp. YGJ3

Author(s) -

Hiroki SAITOU,

Manami Watanabe,

Kiyofumi MARUYAMA

Publication year - 2012

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.120054

Subject(s) - pseudomonas , escherichia coli , recombinant dna , biology , gene , microbiology and biotechnology , hydrolase , pseudomonadaceae , enzyme , genetics , bacteria , biochemistry

Gene phlG encoding 2,4-diacetylphloroglucinol hydrolase was cloned from Pseudomonas sp. YGJ3 and expressed in Escherichia coli. Recombinant PhlG was purified homogeneously. It required 2-mercaptoethanol for stability. Km for 2,4-diacetylphloroglucinol and kcat were determined to be 24 µM and 5.8 s(-1) respectively. CoCl2 specifically and significantly activated PhlG.

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