Purification and Characterization of Multiple Bacteriocins and an Inducing Peptide Produced byEnterococcus faeciumNKR-5-3 from Thai Fermented Fish | Zendy

Naoki Ishibashi | Zendy; Kohei Himeno | Zendy; Koji Fujita | Zendy; Yoshimitsu Masuda | Zendy; Rodney Pérez | Zendy; Takeshi Zendo | Zendy; Pongtep Wilaipun | Zendy; Vichien Leelawatcharamas | Zendy; Jiro Nakayama | Zendy; Kenji Sonomoto | Zendy

Open Access

Purification and Characterization of Multiple Bacteriocins and an Inducing Peptide Produced byEnterococcus faeciumNKR-5-3 from Thai Fermented Fish

Author(s) -

Naoki Ishibashi,

Kohei Himeno,

Koji Fujita,

Yoshimitsu Masuda,

Rodney Pérez,

Takeshi Zendo,

Pongtep Wilaipun,

Vichien Leelawatcharamas,

Jiro Nakayama,

Kenji Sonomoto

Publication year - 2012

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.110972

Subject(s) - bacteriocin , enterococcus faecium , listeria , lactobacillus sakei , listeria monocytogenes , peptide , antimicrobial , biology , fermentation , microbiology and biotechnology , antimicrobial peptides , bacteria , chemistry , biochemistry , lactobacillus , antibiotics , genetics

Enterocins NKR-5-3A, B, C, and D were purified from the culture supernatant of Enterococcus faecium NKR-5-3 and characterized. Among the four purified peptides, enterocin NKR-5-3A (5242.3 Da) was identical to brochocin A, produced by Brochothrix campestris ATCC 43754, in mature peptides, and its putative synergistic peptide, enterocin NKR-5-3Z, was found to be encoded in ent53Z downstream of ent53A, encoding enterocin NKR-5-3A. Enterocin NKR-5-3B (6316.4 Da) showed a broad antimicrobial spectrum, and enterocin NKR-5-3C (4512.8 Da) showed high activity against Listeria. Enterocin NKR-5-3D (2843.5 Da), showing high homology to an inducing peptide produced by Lactobacillus sakei 5, induced the production of the enterocins. The enterocins showed different antimicrobial spectra and intensities. E. faecium NKR-5-3 concomitantly produced enterocins NKR-5-3A, B, C, and D which probably belong to different classes of bacteriocins. Furthermore, NKR-5-3 production was induced by enterocin NKR-5-3D.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research