Open AccessFunctional Divergence of MYB-Related Genes,WEREWOLFandAtMYB23inArabidopsis
Author(s) -
Rumi TominagaWada,
Yuka Nukumizu,
Shusei Sato,
Tomohiko Kato,
Satoshi Tabata,
Takuji Wada
Publication year - 2012
Publication title -
bioscience biotechnology and biochemistry
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.110811
Subject(s) - myb , biology , arabidopsis , trichome , mutant , phenotype , ectopic expression , functional divergence , gene , transgene , genetics , hypocotyl , microbiology and biotechnology , transcription factor , botany , gene expression , gene family
Epidermal cell differentiation in Arabidopsis is studied as a model system to understand the mechanisms that determine the developmental end state of plant cells. MYB-related transcription factors are involved in cell fate determination. To examine the molecular basis of this process, we analyzed the functional relationship of two R2R3-type MYB genes, AtMYB23 (MYB23) and WEREWOLF (WER). MYB23 is involved in leaf trichome formation. WER represses root-hair formation. Swapping domains between MYB23 and WER, we found that a low homology region of MYB23 might be involved in ectopic trichome initiation on hypocotyls. MYB23 and all MYB23-WER (MW) chimeric transgenes rescued the increased root-hair phenotype of the wer-1 mutant. Although WER did not rescue the gl1-1 no-trichome phenotype, MYB23 and all MW chimeric transgenes rescued gl1-1. These results suggest that MYB23 acquired a specific function for trichome differentiation during evolution.
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