Development of an Enzyme-Linked Immunosorbent Assay System for the Determination of Asymmetric Dimethylarginine Using a Specific Monoclonal Antibody | Zendy

Miyuki Yokoro | Zendy; Makiko Suzuki | Zendy; Machiko YATANI | Zendy; Hiromi Yamashita | Zendy; Yoshitaka Takahashi | Zendy; Hideaki Tsuji | Zendy; Masumi Kimoto | Zendy

Open Access

Development of an Enzyme-Linked Immunosorbent Assay System for the Determination of Asymmetric Dimethylarginine Using a Specific Monoclonal Antibody

Author(s) -

Miyuki Yokoro,

Makiko Suzuki,

Machiko YATANI,

Hiromi Yamashita,

Yoshitaka Takahashi,

Hideaki Tsuji,

Masumi Kimoto

Publication year - 2012

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.110727

Subject(s) - asymmetric dimethylarginine , monoclonal antibody , chemistry , enzyme , arginine , nitric oxide synthase , antibody , microbiology and biotechnology , urine , non competitive inhibition , creatinine , monoclonal , nitric oxide , biochemistry , immunology , biology , amino acid , organic chemistry

We produced a monoclonal antibody (mAb) against N(G),N(G)-dimethyl-L-arginine (asymmetric dimethylarginine: ADMA), an endogenous competitive inhibitor of nitric oxide synthase (NOS), and developed an enzyme-linked immunosorbent assay (ELISA). The competitive ELISA method using the mAb determined 5 nM-100 nM ADMA, and ADMA levels in human plasma and urine were found to be 0.78 µM and 51.3 µmol/g of creatinine respectively.

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