A Secreted Placental Alkaline Phosphatase-Based Reporter Assay System for Screening of Compounds Acting at an Octopamine Receptor Stably Expressed in a Mammalian Cell Line | Zendy

Hiroto Ohta | Zendy; H. Oshiumi | Zendy; Naotaka Hayashi | Zendy; Tetsuya Imai | Zendy; Yoshihisa Ozoe | Zendy; Shigeru Morimura | Zendy; Kenji Kida | Zendy

Open Access

A Secreted Placental Alkaline Phosphatase-Based Reporter Assay System for Screening of Compounds Acting at an Octopamine Receptor Stably Expressed in a Mammalian Cell Line

Author(s) -

Hiroto Ohta,

H. Oshiumi,

Naotaka Hayashi,

Tetsuya Imai,

Yoshihisa Ozoe,

Shigeru Morimura,

Kenji Kida

Publication year - 2012

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.110690

Subject(s) - octopamine (neurotransmitter) , alkaline phosphatase , receptor , reporter gene , placental alkaline phosphatase , biology , biochemistry , microbiology and biotechnology , enzyme , gene , gene expression , serotonin

Octopamine receptors are attractive insecticide targets. To screen compounds acting at octopamine receptors simply and rapidly, we constructed a chemiluminescent reporter gene assay system that detects secreted placental alkaline phosphatase transcriptionally regulated by the cAMP response element for a silkworm octopamine receptor. This system proved useful in high-throughput screening to develop octopamine receptor-specific insecticides.

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