Evaluation of the Use of the TobaccoPR-1aPromoter to Monitor Defense Gene Expression by the Luciferase Bioluminescence Reporter System | Zendy

Sachiko Ono | Zendy; Masahiro Kusama | Zendy; Rieko Ogura | Zendy; Kazuyuki Hiratsuka | Zendy

Open Access

Evaluation of the Use of the TobaccoPR-1aPromoter to Monitor Defense Gene Expression by the Luciferase Bioluminescence Reporter System

Author(s) -

Sachiko Ono,

Masahiro Kusama,

Rieko Ogura,

Kazuyuki Hiratsuka

Publication year - 2011

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.110326

Subject(s) - arabidopsis , luciferase , promoter , bioluminescence , reporter gene , biology , transgene , gene expression , gene , npr1 , regulation of gene expression , gus reporter system , mutant , bioreporter , nicotiana tabacum , bioluminescence imaging , microbiology and biotechnology , genetics , transfection , biochemistry , medicine , heart failure , natriuretic peptide

Because of their marked responsiveness to induction signals, genes encoding pathogenesis-related proteins are used as markers to monitor defense gene expression in plants. To develop a non-invasive bioluminescence reporter assay system, we tested acidic PR-1 gene promoters from tobacco and Arabidopsis. These two promoters share common regulatory elements and are believed to show similar responsiveness to various stimuli but the results of transient expression assays by microprojectile bombardment of various plant cells and npr1 mutant Arabidopsis suggest that the tobacco PR-1a promoter is superior to its Arabidopsis counterpart in terms of responsiveness to salicylic acid treatment. Transgenic Arabidopsis seedlings harboring the tobacco PR-1a promoter fused to firefly luciferase showed marked induction in response to treatment with chemicals that induce defense gene expression in plants. These results suggest that the tobacco PR-1a promoter is applicable in monitoring defense-gene expression in various plant species.

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