Polyhydroxyalkanoate (PHA) Synthesis by Class IV PHA Synthases EmployingRalstonia eutrophaPHB−4 as Host Strain | Zendy

Manami Hyakutake | Zendy; Yuta Saito | Zendy; Satoshi Tomizawa | Zendy; Kouhei Mizuno | Zendy; Takeharu Tsuge | Zendy

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Polyhydroxyalkanoate (PHA) Synthesis by Class IV PHA Synthases EmployingRalstonia eutrophaPHB⁻4 as Host Strain

Author(s) -

Manami Hyakutake,

Yuta Saito,

Satoshi Tomizawa,

Kouhei Mizuno,

Takeharu Tsuge

Publication year - 2011

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.110229

Subject(s) - ralstonia , polyhydroxyalkanoates , strain (injury) , cupriavidus necator , host (biology) , microbiology and biotechnology , chemistry , bioplastic , stereochemistry , biology , bacteria , biochemistry , genetics , enzyme , anatomy , ecology

Class IV polyhydroxyalkanoate (PHA) synthase from Bacillus cereus YB-4 (PhaRC(YB4)) or B. megaterium NBRC15308(T) (PhaRC(Bm)) was expressed in Ralstonia eutropha PHB(-)4 to compare the ability to produce PHA and the substrate specificity of PhaRCs. PhaRC(YB4) produced significant amounts of PHA and had broader substrate specificity than PhaRC(Bm).

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