Kinetic Resolution of 3-Fluoroalanine Using a Fusion Protein ofD-Amino Acid Oxidase withVitroscillaHemoglobin | Zendy

Young-Man Seo | Zendy; YongHo Khang | Zendy; Hyungdon Yun | Zendy

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Kinetic Resolution of 3-Fluoroalanine Using a Fusion Protein ofD-Amino Acid Oxidase withVitroscillaHemoglobin

Author(s) -

Young-Man Seo,

YongHo Khang,

Hyungdon Yun

Publication year - 2011

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.110122

Subject(s) - oxidase test , d amino acid oxidase , fusion protein , chemistry , escherichia coli , fusion , biochemistry , amino acid , recombinant dna , enzyme , gene , linguistics , philosophy

In this study, a fusion protein (VHb-DAAO) of D-amino acid oxidase (DAAO) with Vitreoscilla hemoglobin (VHb) was functionally expressed in Escherichia coli and purified. The k(cat) value VHb-DAAO (47.1 s⁻¹) towards rac-3-flouroalanine was about 2-fold higher than that of DAAO (21.9 s⁻¹). rac-3-Flouroalanine (500 mM) was kinetically resolved into (R)-3-fluoroalanine with high enatiomeric excess (>99%) by VHb-DAAO with about 52% conversion.

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