The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA fromPyrococcus horikoshiiOT3 | Zendy

Tadashi HARA | Zendy; Atsushi Terada | Zendy; H Yamaguchi | Zendy; Takashi Nakashima | Zendy; Yoshimitsu Kakuta | Zendy; Makoto Kimura | Zendy

Open Access

The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA fromPyrococcus horikoshiiOT3

Author(s) -

Tadashi HARA,

Atsushi Terada,

H Yamaguchi,

Takashi Nakashima,

Yoshimitsu Kakuta,

Makoto Kimura

Publication year - 2011

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.110110

Subject(s) - pyrococcus horikoshii , rnase p , rna , transfer rna , mutant , biology , cleavage (geology) , catalysis , biochemistry , chemistry , enzyme , gene , paleontology , fracture (geology)

We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.

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