Open AccessIn Vitroandin VivoMelanogenesis Inhibition by Biochanin A fromTrifolium pratense
Author(s) -
Victor C. Lin,
HsiouYu Ding,
Pin-Chin TSAI,
JiumnYih Wu,
Yen-Hsing Lu,
TeSheng Chang
Publication year - 2011
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.100878
Subject(s) - biochanin a , in vivo , tyrosinase , in vitro , zebrafish , pharmacology , melanin , hyperpigmentation , chemistry , melanoma , traditional medicine , biology , medicine , biochemistry , genistein , endocrinology , enzyme , cancer research , gene , microbiology and biotechnology , daidzein
Our previous study showed that a methanol extract from Trifolium pratense exerted potent inhibitory activity on melanogenesis in mouse B16 melanoma cells. In the present study, the active compound in this Chinese herb extract was isolated and identified as biochanin A by mass spectrum, (1)H-NMR, and (13)C-NMR analysis. The inhibitory effects of biochanin A on melanogenesis were investigated in vitro in cultured melanoma cells and in vivo in zebrafish and mice. Biochanin A dose-dependently inhibited both melanogenesis and cellular tyrosinase activity in B16 cells and in zebrafish embryos. Application of a cream containing 2% biochanin A twice daily to the skin of mice also increased the skin-whitening index value after 1 week of treatment, and the increase continued for another 2 weeks. Biochanin A was confirmed as a good candidate for use as a skin-whitening agent in the treatment of skin hyperpigmentation disorders.
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