Open AccessCharacterization of Recombinant β-Amylases fromOryza sativa
Author(s) -
Tomojiro Koide,
Yasuo Ohnishi,
Sueharu Horinouchi
Publication year - 2011
Publication title -
bioscience biotechnology and biochemistry
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.100872
Subject(s) - oryza sativa , recombinant dna , amylase , gene , complementary dna , escherichia coli , biology , chemistry , biochemistry , enzyme
Four putative β-amylase genes found in the Oryza sativa cDNA sequence database (KOME) were expressed in Escherichia coli. Recombinant proteins from two of these genes showed β-amylase activity. Similarly to β-amylases from other plants, the optimum pH of the recombinant rice β-amylases was about 5.5-6.0, but they exhibited inferior heat stability to soybean β-amylase.
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