Selective, High Conversion of D -Glucose to 5-Keto-D -gluoconate by Gluconobacter suboxydans
Author(s) -
Yoshitaka Ano,
Emiko Shinagawa,
Osao Adachi,
Hirohide Toyama,
Toshiharu Yakushi,
Kazunobu Matsushita
Publication year - 2011
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.100701
Subject(s) - yield (engineering) , oxidizing agent , chemistry , biochemistry , catalysis , organic chemistry , materials science , metallurgy
Selective, high-yield production of 5-keto-D-gluconate (5KGA) from D-glucose by Gluconobacter was achieved without genetic modification. 5KGA production by Gluconobacter suffers byproduct formation of 2-keto-D-gluconate (2KGA). By controlling the medium pH strictly in a range of pH 3.5-4.0, 5KGA was accumulated with 87% conversion yield from D-glucose. The pH dependency of 5KGA formation appeared to be related to that of gluconate oxidizing activity.
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