A New Beneficial Mutation inPseudomonassp. 61-3 Polyhydroxyalkanoate (PHA) Synthase for Enhanced Cellular Content of 3-Hydroxybutyrate-Based PHA Explored Using Its Enzyme Homolog as a Mutation Template | Zendy

Fumi Shozui | Zendy; Jian Sun | Zendy; Yuyang Song | Zendy; Miwa Yamada | Zendy; Kohei Sakai | Zendy; Ken’ichiro Matsumoto | Zendy; Kazuma Takase | Zendy; Seiichi Taguchi | Zendy

Open Access

A New Beneficial Mutation inPseudomonassp. 61-3 Polyhydroxyalkanoate (PHA) Synthase for Enhanced Cellular Content of 3-Hydroxybutyrate-Based PHA Explored Using Its Enzyme Homolog as a Mutation Template

Author(s) -

Fumi Shozui,

Jian Sun,

Yuyang Song,

Miwa Yamada,

Kohei Sakai,

Ken’ichiro Matsumoto,

Kazuma Takase,

Seiichi Taguchi

Publication year - 2010

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.100224

Subject(s) - polyhydroxyalkanoates , escherichia coli , pseudomonas , enzyme , mutation , overproduction , atp synthase , biochemistry , recombinant dna , biology , chemistry , microbiology and biotechnology , bacteria , gene , genetics

A newly isolated mutation (Gln508Leu) and a combination of it with previously discovered beneficial mutations in polyhydroxyalkanoate synthase 1 from Pseudomonas sp. 61-3 were found to enhance the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3HB-co-3-hydroxyalkanoate)s in recombinant Escherichia coli.

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