A Real-Time SUMO-Binding Assay for the Analysis of the SUMO-SIM Protein Interaction Network | Zendy

N Tanaka | Zendy; Hisato Saitoh | Zendy

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A Real-Time SUMO-Binding Assay for the Analysis of the SUMO-SIM Protein Interaction Network

Author(s) -

N Tanaka,

Hisato Saitoh

Publication year - 2010

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.100082

Subject(s) - gene silencing , identification (biology) , computational biology , chemistry , ligand binding assay , microbiology and biotechnology , binding site , biology , gene , genetics , receptor , botany

SUMO-interacting motifs (SIMs) play a central role in the fate of SUMO-modified proteins. Here we report a real-time SUMO-binding assay. It can be applied to the identification of SIMs and to screening for the identification of novel SUMO-binding proteins. Using this assay, we investigated the SIMs in SETDB1 and MCAF1 to gain insight into the assembly of SETDB1-MCAF1-mediated gene silencing.

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