Improvement of Coenzyme Q10Production by Increasing the NADH/NAD+Ratio inAgrobacterium tumefaciens | Zendy

Bong-Seong KOO | Zendy; Yun-Jung GONG | Zendy; Sang Yong Kim | Zendy; ChanWha Kim | Zendy; HyeonCheol Lee | Zendy

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Improvement of Coenzyme Q₁₀Production by Increasing the NADH/NAD⁺Ratio inAgrobacterium tumefaciens

Author(s) -

Bong-Seong KOO,

Yun-Jung GONG,

Sang Yong Kim,

ChanWha Kim,

HyeonCheol Lee

Publication year - 2010

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.100034

Subject(s) - agrobacterium tumefaciens , nad+ kinase , cofactor , production (economics) , chemistry , physics , transformation (genetics) , biochemistry , enzyme , gene , macroeconomics , economics

Previously screened CoQ(10)-overproducing Agrobacterium tumefaciens A603-35 showed a relatively high NADH/NAD(+) ratio (1.1), as compared to parental strain C58 (0.2) when we increased the expression levels of NADH-generating enzymes. Also, the intracellular NADH/NAD(+) ratio showed a positive correlation with the CoQ(10) content in A603-35. Overexpression of glyceraldehyde 3-phosphate dehydrogenase in A603-35 shifted the NADH/NAD(+) ratio at 48 h from 0.8 to 1.2, and thus the CoQ(10) content in flask culture increased from 2.16 to 3.63 mg/g DCW. Due to the addition of hydroxybutyrate to the culture media, the intracellular NADH/NAD(+) ratio in A603-35-gapA shifted from 1.2 to 1.4, which led to an increase CoQ(10) content (5.27 mg/g DCW).

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